Perfecta3D® Hanging Drop Plates Protocol: 384-Well Plate Spheroid Transfer and Harvesting

Spheroids can be harvested from either the top or bottom side of the Perfecta3D Hanging Drop Plate. Once harvested, the spheroids can be processed for further culturing (e.g. differentiation, invasion assay, etc.) or analysis (e.g. flow cytometry, immunofluorescence staining, histological staining, confocal microscopy, etc.). This note presents a protocol for the transfer and harvesting of spheroids from a 384- Perfecta3D Hanging Drop Plate.