Practical Application of Simoa™ HD-1 Analyzer for Ultrasensitive Multiplex Immunodetection of Protein Biomarkers

This white paper presents the use of Simoa™ technology in ultrasensitive multiplex immune detection of protein biomarkers. In this approach, also known as Digital ELISA, single protein molecules are captured on antibody-coated, paramagnetic beads. The captured proteins are labeled with an enzyme label, and single beads are isolated and sealed in arrays of femtoliter wells in the presence of enzyme substrate before being detected on an uncooled CCD camera using a white light excitation source.