Production of Human Induced Pluripotent Stem CellDerived Cortical Neurospheres in the DASbox Mini Bioreactor System

Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up. We used a DASbox Mini Bioreactor System for the generation of human induced pluripotent stem cell (hiPSC) spheroids and subsequent neural differentiation. Results from three independent bioprocesses demonstrate the reproducibility of the neural differentiation protocol. In a single DASbox Mini Bioreactor, we obtained 2 x 10⁸ cells, demonstrating the potential of stirred-tank bioreactors for the generation of high numbers of iPSCderived neurospheres.