Proliferation and Cell Cycle Analysis Using the NovoCyte Flow Cytometer

Eukaryotic cell division is an evolutionary process where specific cellular components are duplicated in order to be divided equally amongst daughter cells. The precise timing and regulation of cell division is essential to many normal physiological processes and studying the molecular mechanisms regulating cell division is central to understanding both physiological and pathophysiological events, such as cancer. This application note demonstrates the analysis of specific phases of the cell cycle by treating Jurkat T cells with different compounds known to induce growth arrest at G1 and G2/M.