Propagation of Human Pluripotent Stem Cells in Three Dimensional Culture Using Alvetex®Strata

amsbio has developed a strategy that enables the continuous propagation and maintenance of cells in three-dimensional (3D) culture. The technology relies on culturing cells continuously on the surface of Alvetex®Strata, a highly porous membrane presented in a well insert format. As proof of concept, the propagation of human pluripotent stem cells in 3D is demonstrated over many passages. This application note demonstrates how using Alvetex®Strata offers a new approach to cultivating cells continuously in 3D to achieve notable improvements in cell growth and function. It can be used to routinely maintain proliferative cells with a 3D structural phenotype in preparation for their use in down-stream assays that involve 3D cell growth.