Protein Sample Preparation in Eppendorf Tubes® 5.0 mL

Analysis of proteins from cultured cells often requires sample processing at a larger volume scale. Examples include isolation of cell fractions from larger homogenates, determination of enzyme activities as well as harvesting of proteins from sparsely growing cells. This application note investigates the performance of the Eppendorf 5.0 mL tube format with a comparative analysis for isolation and preparation of protein samples using different tube volume formats.