Pushing the Boundaries of 3D Microtissue Analysis using High Content Imaging

3D cell culture methods are widely accepted as being more physiologically relevant than conventional 2D cell culture methods, and are believed to improve the prediction of drug candidates at an early stage in the drug development process. The visualization of 3D structures is challenging, for example when imaging microtissues there is light scattering and absorption which prevents imaging deep into the center of the microtissue. This application note demonstrates the imaging of microtissue using the Opera® High Content Screening System (equipped with a water objective lens) in combination with a microtissue pretreatment.