Qualitative and Quantitative Characterization of Therapeutic Antibody using High Speed and High Resolution Mass Spectrometry

Mass Spectrometry has become a critical tool for structural characterization of therapeutic antibodies. Qualitative analysis of intact protein, amino acid sequence, enzymatic peptides, disulfide linkages, carbohydrates and various post translational modifications can be done using high resolution MS and MS/MS data acquisition. For high accurate and high throughput data analysis, we need an optimized data acquisition method and sophisticated data processing tools. This poster demonstrates the optimization of a LC/MS/MS data acquisition method for antibody characterization and established a new data processing pipeline using XICs.