Quantification of Efflux Transporter Activity in 3D Human Liver Microtissues

A functional impairment of hepatobiliary transporters such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP2) are strongly associated with an increased risk of liver injury. The use of 3D human liver microtissues for assessing hepatobiliary transporter function is presented in this poster. Confocal imaging was used to assess BSEP-mediated efflux of cholyl-lysyl-fluorescein (CLF) and MRP2-mediated efflux of 5-chloromethylfluorescein diacetate (CMFDA) into the bile canaliculi. 3D InSight Human Liver Microtissues were produced with InSphero’s propietary production process and maintained in GravityTRAP plates until further analysis.