Quantification of MMP Activity and Inhibition in a 3D Tumor Invasion Model Using the Cytation™ 5 Cell Imaging Multi-Mode Reader

Metastasis, the spread of cancer cells from the primary tumor to secondary locations within the body, is linked to approximately 90% of cancer deaths. MMP-14 is an enzyme crucial for basement membrane and interstitial matrix degradation, and has been shown to play a critical role in conferring cells with the ability to penetrate the extracellular matrix. This application note demonstrates the ability to image and quantify MMP-14 activity, in addition to tumor invasion, using a 3D tumoroid cell model.