Quantitation of Pathogen Copy Numbers using CE-IVD-Marked artus LC PCR Kits and the LightCycler® 2.0 Instrument

This application note details the protocol for the quantitation of pathogen copy numbers. Due to its exceptional sensitivity, specificity, and high dynamic range, quantitative, real-time polymerase chain reaction (qPCR) is the essential technique for nucleic acid quantitation. qPCR allows data analysis by comparison of individual reactions, with the same amplification efficiency, at the beginning of the logarithmic-linear phase.