Quantitative Analysis of 3-Color Fluorescent Western Blots with the FluorChem^® Q

This application note describes a Multicolour Fluorescent Western Blot allowing the detection of multiple proteins on the same blot, without stripping and reprobing, with final quantative, straightforward and accurate analysis. The FluorChem Q, a CCD-based detection platform is able to rapidly and easily perform traditional chemiluminescent and multicolour fluorescent detection. Combined with AlphaView™ Q software and FluorChem Q, the process from image acquisition to image analysis is simplified, suggesting a tool for imaging and analysing multicolour Westerns.