Rapid Hit Confirmation and Early hERG Liability Testing Using the IonWorks HT System

This application note illustrates how the IonWorks HT System can be used to rapidly confirm hits from higher-throughput assays as well as perform early hERG liability testing for ion channel targets. Twenty two compounds from Library of Pharmacologically Active Compounds were identified with apparent inhibitory activity on the cardiac sodium channel (Nav1.5) using the FLIPR® Membrane Potential Assay Kit. These 22 compounds were then assayed at a single dose using the IonWorks® HT System and one compound (rimcazole) was confirmed as having inhibitory activity on the Nav1.5 channel.