Recombinase Polymerase Amplification: An Isothermal Technique Suitable for a Range of Applications - Evaluation of RPA Volume Tolerance

TwistDx’s proprietary Recombinase Polymerase Amplification (RPA) method addresses many of the challenges faced by PCR methods through a number of benefits, such as the low running temperature. In this application note, RPA performance is demonstrated in reactions established in significantly different total reaction volumes and performed in standard PCR tubes (0.2ml), without oil overlays or any other specialized consumables.