Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System

Peptide mapping is one of the routine methods for biotherapeutics characterization. This technique, combined with mass spectrometry, is utilized in research environments for the determination of the primary sequence of an antibody or the identification of post-translational modifications. UV is often used as a unique detection tool, for instance in stability studies. The data interpretation of these experiments is based on retention times as qualitative and peak area as quantitative information. In this application note, the separation of peptides obtained from a bovine serum albumin (BSA) digest is demonstrated. The retention time and peak area precisions are evaluated for repeated injections.