Revealing cellular metabolic phenotype and function using Agilent XF substrate oxidation stress tests

In this application note, Agilent Technologies showcases its Seahorse XF technology, which enables real-time measurement of energy metabolism in live cells, offering vital functional insights related to cellular health and fitness. The Agilent Seahorse XF Substrate Oxidation stress test provides essential metrics for investigating specific mitochondrial substrates that impact cellular phenotype and function. The study includes an assay strategy overview, data examples, and guidance on applying these methods in cancer and immunological cell metabolism research for drug discovery.