RNA Extraction From Ixoidid Ticks With Precellys Evolution

Tick-borne disease surveillance is becoming increasingly important as zoonotic tick-borne pathogens are recognized to affect man and wild and domestic animals worldwide. Dragging vegetation for questing ticks are often employed where ticks are present and provides information on habitats and seasonal life stage distributions. This study aimed to provide an estimate of the distribution of life cycle stages during periods when ixoidid ticks are active and their infection status with SFTS virus. The Precellys24 was used to extract RNA from pools of ticks larvae, nymphs and adults in order to examine the infection status with severe fever with thrombocytopenia syndrome (SFTS) virus using RT-PCR.