RNA Isolation from 3D Microtissues Using the Promega ReliaPrep™ RNA Tissue Miniprep System

This application note details a protocol to isolate total RNA from as little as 1 microtissue (~ 1000 cells) using the ReliaPrep™ RNA Tissue Miniprep System. Isolation of high quality total RNA from cultured cells is important for various analyses in cell-based drug discovery and toxicity. Analytical applications such as quantitative real time PCR or microarray analysis depend on robust RNA isolation procedures.