Screening and semi-quantitation of 212 fentanyl analog compounds by the Orbitrap Exploris 120 mass spectrometer

In this application note from Thermo Fisher Scientific, explore a method for fentanyl screening and semi-quantitation in urine for all 212 fentanyl analog compounds contained in the Fentanyl Analog Screening (FAS) Kit and Emergent Panels. Sample preparation includes simple 20× dilution in water, quantitation by full high-resolution accurate mass (HRAM) MS scan, and confident confirmation by retention time, accurate m/z, isotope pattern matching to calculated theoretical isotope pattern, and matching experimentally collected MS² spectra to an in-house MS² spectral library.