Screening for Use-Dependent Blockers of NaV1.5 Channels on the IonWorks Barracuda Automated Patch Clamp System

This application note describes the design and validation of a cell based assay for the NaV1.5 channel, using the IonWorks Barracuda® Automated Patch Clamp System. A single concentration mock screen of known sodium channel blockers was set up to identify the location of the hits in a 384 compound plate. Follow-up experiments on the hits were then performed to identify use- and state-dependent blockade using a train of depolarizing pulses.