Screening of GPCR-Antagonists Using the Calcium Mobilization Assay

This application note describes a study in which three compounds were screened for P2Y₂ receptor inhibition using a calcium mobilization assay with two different fluorescent dyes. The fluorescence intensity signal was measured using a TriStar² LB 942 plate reader. The basic principle behind the assay is described and the materials and methods detailed. It was found that the TriStar² LB 942 could accurately screen test compounds for antagonist activity with precision and high reproducibility.