Selecting Buffers to Remove Uncertainty in Tryptic Digestion

Protein digestion is a fundamental technique employed in biopharmaceutical and proteomic applications. It is used to analyze the sample and identify structural features or post-translational modifications (PTMs). Despite its widespread use, protein digestion still provides many analytical challenges. This application note demonstrates how Thermo Scientific™ SMART Digest™ kits remove uncertainty associated with conventional solution-based tryptic digestion protocols, resulting in higher reproducibility and sample characterization.