Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit

Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However for biological applications involving small samples, such as next-generation sequencing and quantitation of DNA amplification products, sensitive methods are needed.

In this application note, Molecular Devices demonstrates how the Molecular Devices SpectraMax^® microplate readers and the Quant-iT PicoGreen assay can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA.