Sensitivity of a Combined DLS/Raman Technique to Estimate the Conformational and Colloidal Stability of Protein Therapeutics

This white paper demonstrates that the combination of DLS/Raman can easily be used for the elucidation of protein conformation (Raman) and size (DLS) changes when exposed to stressors. The lower concentration limit for Raman detection, traditionally only used to study high concentration samples, has been evaluated here by performing thermal ramping experiments. The study shows that a model protein, lysozyme, can be studied at concentrations as low as 3 mg/mL, with the identification of secondary and tertiary structural markers. The combination of these complementary techniques can allow for the understanding of protein unfolding and aggregation pathways at both low and high concentrations.