Separation of deamidated peptides with an Agilent AdvanceBio Peptide Plus column

27 Jun 2021

Deamidations of glutamine and asparagine are among the most common degradations affecting proteins. This application note demonstrates that a charged surface C18 column greatly enhances selectivity for deamidated variants of peptides versus their unmodified forms when compared to a traditional C18 column.

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