Sf-9 Insect Cell Culture Using a New Brunswick™ CelliGen® 310 Bioreactor: Using Headspace Air Overlay for Reduced dCO2

This study describes a simple procedure for improving insect cell yields in a benchtop cell culture bioreactor. Here, yields of Spodoptera frugiperda (Sf-9) cells were increased by nearly 29 % through monitoring dissolved carbon dioxide (dCO2) levels in the culture and adding air to the vessel headspace to reduce dCO2. The method can also be used to maximize yields in a wide range of mammalian cell types.