Simplified workflow using automated instrument setup and compensation on the BD FACSLyric Flow Cytometer

In this application note, immunophenotyping results from three to six 10-color BD FACSLyric™ Flow Cytometers were evaluated over two months. Overall performance was assessed using a multicolor assay for T, B and NK cells in peripheral blood from HIV patients and controls. The results show that the BD FACSLyric™ System provides unique tools to the clinical user to help achieve reproducible and consistent results across time and instruments. The application note demonstrates the ease of use, reproducibility of automated setup and compensation, and resolution sensitivity of the BD FACSLyric™ Flow Cytometer platform. These features may be useful for generating consistent results for longitudinal or multisite studies in which the same assay is used to acquire data on different days, on different instruments or by many operators with varying levels of expertise.