Solid phase extraction of fentanyl analogues in urine

The detection of synthetic opioids including fentanyl in biological samples is crucial in clinical and forensic toxicology. Analysis techniques, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) enable multiplexed detection of fentanyl analogues with high selectivity and sensitivity. However, achieving high throughput with accurate quantification requires sophisticated sample preparation. Automated solid phase extraction (SPE) applied to fentanyl extraction with HP-SCX sorbent, offering reversed phase and strong cation exchange chemistry, addresses this challenge. Tecan highlights an automated workflow using the Resolvex^® i300 and HP-SCX Narrow Bore Extraction columns (NBE columns™) to extract 23 fentanyl analogues from human urine efficiently.