Solving the Aggregation Problem of Human Embryonic Kidney 293 Cells Using the New Brunswick™ S41i CO₂ Incubator Shaker

This application note shows a successful example of the adaptation of a human recombinant protein-expressing HEK293 cell line from serum-supplemented attachment culture to serum-free single cell suspension culture using the New Brunswick S41i CO₂ incubator shaker. This adapted HEK293 cell line behaves like a typical aggregationless suspension cell line.