Solving the Controversy Surrounding the Marker Fragment at 193 for the Positive Identification of 8-iso Prostaglandin F2α

This poster describes a follow-up study using MS/MS from high resolution FT-ICR Mass Spectrometry to investigate the structural conundrum experienced when identifying 8-iso prostaglandin. F2-isoprostanes, in vivo, is an important cell membrane component and a biomarker of oxidative stress. There are four classes of isomers, each with 8 racemic diastereomers. Thus, 64 different isomer compounds can be produced through this mechanism.