Streamlined workflow for absolute quantitation of therapeutic monoclonal antibodies using Promise Proteomics mAbXmise kits and a TSQ Altis Plus mass spectrometer

For advanced productivity and efficacy in clinical research testing, rapid and high-throughput testing, automation, and high-end instrumentation requires a reliable and robust workflow for quantitative analysis. In this application note, Thermo Fisher Scientific presents the first commercial ready-to-use kits to simultaneously quantify the signature peptides of multiple therapeutic monoclonal antibodies in human serum in a streamlined and confident way using Promise Proteomics mAbXmise™ kits and the TSQ Altis™ Plus mass spectrometer. This process can also be applied to therapeutic drug monitoring (TDM) during patient therapy. Used for analyzing the concentration of drugs in blood, plasma and serum to optimize dosing in therapeutic monoclonal antibodies (mAbs). Good results have been speculated with LC-MS/MS analysis using the TSQ Altis Plus mass spectrometry (MS) which measures highly complex biological proteins qualitatively and quantitatively for quantitative analysis of mAbs.