Successive Proximity Extension Amplification Reaction (SPEAR) and its two-factor authentication mechanism

Successive Proximity Extension Amplification Reaction (SPEAR) is an innovative assay technology that enhances protein detection sensitivity using a unique two-factor authentication mechanism. By leveraging nucleic acid amplification, SPEAR minimizes background noise while maintaining high specificity. This wash-free, homogeneous format enables ultra-sensitive biomarker quantification for clinical and research applications, particularly in neurodegenerative disease detection.