Superior High-throughput Genotyping with KlearKall Master mix and BHQplus probes

High-throughput genotyping of SNPs (single nucleotide polymorphisms) is used to analyze large numbers of human samples in genome-wide association studies and for validating any disease-linked genetic variants found. Analysis of large numbers of samples accurately and efficiently is fundamental to this process. In agriculture, using SNPs as desirable trait markers is a powerful tool to accelerate breeding programs for crop improvement. Crude DNA extracts are commonly used for this as they are rapid and cost effective to prepare. However, crude extracts produce poor quality DNA and carry debris and endogenous PCR inhibitors. This application note demonstrates the use of an optimized PCR mix high-quality, high-throughput endpoint genotyping with hydrolysis probes in low (1 μL) reaction volumes.