The effect of Blebbistatin and Cytochalasin-D on the migration capacity of mouse embryonic fibroblasts

Wound healing assays are commonly used to understand the migratory potential of cells in vitro, however, the quality of their results depends on the materials used and the user experience, which can make these assays imprecise and inconsistent.

In this application note, CytoSmart demonstrates how to solve these issues using the Autoscratch™ Wound Making Tool to create a uniform and identical scratch areas in every sample. The cloud-based CytoSMART^® Scratch Algorithm accurately determined the scratch area of each well in high-resolution scans that were made every hour for a 24-hour period.