The FluorChem Q^® and ECL Plex^™: Sensitive and Quantitative Imaging of Multicolor Fluorescent Westerns

This application note outlines a technique which facilitates quantification of proteins via the use of an internal reference protein removing the need to strip and reprobe a blot. Cell Biosciences describe the sensitivity and linear range which can be achieved in using the FluorChem Q with ECL Plex labeled Westerns, and the compatibility of the FluorChem Q with multicolour Western blots.