The Importance of Coverage Uniformity Over On-Target Rate for Efficient Targeted NGS

In this paper, Twist Bioscience use ranges of on-target rate and uniformity typical of commercial exome kits to mathematically model the relative impacts of both metrics on overall efficiency. It's demonstratd that, though most commercial probe panels cite only on-target rate in their specifications, uniformity has a more significant contribution to the efficiency of targeted sequencing.