The Solution Conformation of Novel Antibody Fragments Studied using the Proteomelab Xl-A Analytical Ultracentrifuge

In this study the monodispersity and absence of selfassociation phenomena of Fab’ and F(ab’)2 solutions are strongly indicated by sedimentation velocity and sedimentation equilibrium experiments. Cross-linking of novel Fab’ fragments has been a recent attempt to improve monoclonal antibodies in their use as radiolabeled diagnostic markers. In order to properly understand the function of these new antibody fragments in vivo, application of the ProteomeLab XL-A analytical ultracentrifuge was utilized to gain an appreciation of their size, self-association behavior (or preferably lack of) and solution conformation.