The use of Molecular Devices’ ImageXpress® for Cardiovascular Research, Toxicity, and Drug Discovery in Human IPS-Derived Cardiomyocytes

This application note describes how Molecular Devices’ ImageXpress® Velos and ImageXpress® Micro High Content Screening Systems were used to obtain and analyze cell images for overall viability and early damage to pluripotent stem cells, as indicated by disruption of the mitochondrial membrane. Induced pluripotent stem cell (iPSC)- derived human cardiomyocytes are emerging as a biorelevant model system for testing toxicity and cardioprotective effects of drug candidates and developing drugs. These experiments illustrate the use of high content imaging for powerful and automated assessment of toxicity and to determine the functional effects of compounds on living human cardiac cells.