Therapeutic nucleic acid application notebook

Oligonucleotide synthesis includes the repeated use of protection groups that need to be removed after the last cycle. Impurities emerge through the faulty addition of two similar nucleotides in one cycle or a failed coupling of the last nucleotide. Chromatography can also separate oligonucleotides carrying protection groups from deprotected oligonucleotides and it can even facilitate the cleavage and removal of protection groups. In this notebook from Tosoh Bioscience, explore several applications for the purification of synthetic oligonucleotides.