Tumor Spheroid Brightfield Analysis

A growing body of evidence suggests that more relevant and translational observations can be made with micro-tissues and organoids compared to 2D monolayer cell models. This application note describes methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte^® S3 Spheroid software module. It demonstrates that these assays are flexible, simple to run and provide automated and direct measures of tumor size in real time.