Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone-Modifying Enzyme Complex

Methylation of arginine residues by protein arginine methyltransferases (PRMTs) is an underexplored component of the histone code by epigenetic regulation. The histone code encompasses an exceedingly complex combination of post-translational modifications (PTMs) of histone residues that marks and directs regulation of the underlying genome. This application note describes the use of a novel peptide array to study the substrate specificity of a critical enzyme complex.