User guide to simplified & optimized multiplexing cell analysis

Cell-based assays make up a large part of the workload for many laboratories. However, the major problem cell biologists often encounter when conducting in vitro cell-based assays is the lack of reproducibility of data. This can result from a range of factors, including physiological conditions, the use of end-point assays, and limitations in cell imaging technology.

In this eBook, learn how advances in cell-based technology such as real-time live-cell imaging and image-based cytometry can bring higher levels of automation, reproducibility, and flexibility to your cell-based research.

The eBook covers optimized multiplexing cell analysis protocols for:

• Cell counting and confluence analysis
• Imaging-based analysis of cellular transfection efficiency
• Imaging-based assessment of cell viability and cytotoxicity
• Analysis of cell death
• Image-based detection of apoptosis
• Normalizing cellular fluorescence
• Monitoring intracellular calcium
• Real-time experimental control for cell-based assays
• Real-time experimental control for live cell fluorescence reporter applications