Using Inverted SPIM (iSPIM) to Study Neural Development in <i>C. elegans</i>

This application note describes the use of a light sheet fluorescence microscopy technique, Inverted Selective Plane Illumination Microscopy (iSPIM), to image the neural development of the nematode C. elegans. iSPIM aenables very fast non-invasive imaging of living samples, facilitating the inspection of live neurodevelopmental events and paving the way for a 4D dynamic neurodevelopmental atlas.