Using MST to Analyze the Binding of the β-Lactamase TEM1 to BLIP

The analysis of protein-protein interactions plays an important role in understanding many biological processes. MicroScale Thermophoresis (MST) permits a robust and fast analysis of protein-protein interactions even in highly complex biological liquids. In this application note, the binding of TEM1 β – Lactamase to BLIP (β – Lactamase Inhibitory Protein) is investigated using MicroScale Thermophoresis. The experiments were performed as in 50% mammalian cell lysate, as well as in standard buffer. The TEM1-BLIP system is very well characterized by various biophysical methods, such as Surface Plasmon Resonance (SPR).