Validated 10-color T Cell Panel Using the Attune NxT Flow Cytometer

Multicolor immunofluorescence panels are widely used in flow cytometry and are often difficult and time-consuming to develop. This application note discusses Optimized Multicolor Immunofluorescence Panel (OMIP) publication 009 which addresses characterizing the immunological response of human T cells and the importance of antibody titration and the optimization of antibody concentration in panel testing. The results within the OMIP-009 were analyzed using Attune NxT Flow Cytometer and Attune NxT Software version 2.5.