Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging

This application note demonstrates a novel 3D wound healing assay model that can overcome 2D assay limitations. Wound healing, both acute and chronic, involves a complex internal and external choreography of signaling, in addition to interactions with neighboring cells and the surrounding environment or external stimuli, and cell migration. Historically, most wound healing assays use a scratch technique but this method is limited due to the lack of biomimetic environment, in vivo-like architecture and multi-cellular network.