Viral titer determination

Recombinant adeno-associated virus (AAV) is rapidly becoming the vector of choice for human gene therapy applications due to the ability to achieve prolonged gene expression in a variety of tissues without a significant immune response or toxicity. Bio-Rad has discovered a number of novel AAV serotypes with enhanced gene expression and alternate tissue tropisms and has pioneered the study of these vectors for gene delivery. In this application note, Bio-Rad contrasts and compares the benefits of Droplet Digital PCR (ddPCR) versus quantitative PCR (qPCR) in the determination of adeno-associated virus (AAV) vector genome titers.