Western Blot - Procedure for Simultaneous Extraction of Cytoplasmic, Nuclear and Chromatin Proteins with Bioruptor

The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor®, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins.