Western Blot Protein Detection and Quantitation Based on Europium Labeled Proteins Using a Plate Reader

Protein detection is an important task for pharmaceutical and clinical research today and Western Blots (WB) are one of the most common methods employed for this purpose. Here we report a novel system for WB membrane protein analysis that is incorporated into a SpectraMax® Multi-Mode Microplate Reader. The method does not involve enzyme detection, and the Eu-chelates are resistant to photo-bleaching so the signal remains stable for long periods of time (weeks to months).