Ideal for low-expressing protein targets, the LOBSTR E. coli expression strain allows for one-step polyhistidine-tag affinity purification by eliminating the two major E. coli contaminants. Traditionally, a major drawback to using polyhistidine-tag affinity purification for proteins expressed in E. coli is contamination resulting from the presence of two naturally histidine-rich proteins in E. coli, ArnA and SlyD. LOBSTR (l…
The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR-Grade Nucleotides.FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incuba…
The FastStart High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify fragments up to 5 kb. Hot Start PCR Multiplex PCR RT-PCR GC-rich template amplification Difficult, challenging PCRs The FastStart High Fidelity PCR System is the product of choice for multiplex PCR. Please see the onl…
